Presumed pluripotency markers UTF-1 and REX-1 are expressed in human adult testes and germ cell neoplasms
DM Kristensen, JE Nielsen, NE Skakkebaek… - Human …, 2008 - academic.oup.com
Human reproduction, 2008•academic.oup.com
Abstract BACKGROUND UTF-1 and REX-1/ZFP42 are transcription factors involved in
pluripotency. Because of phenotypic similarities between pluripotent embryonic stem cells
and testicular germ cell tumours (TGCT) and the derivation of pluripotent cells from testes,
we investigated the expression of UTF-1 and REX-1 during human gonadal development
and in TGCT. METHODS Expression of UTF-1 and REX-1 was studied in 52 specimens from
human gonadal development and in 86 samples from TGCT. RESULTS UTF-1 and REX-1 …
pluripotency. Because of phenotypic similarities between pluripotent embryonic stem cells
and testicular germ cell tumours (TGCT) and the derivation of pluripotent cells from testes,
we investigated the expression of UTF-1 and REX-1 during human gonadal development
and in TGCT. METHODS Expression of UTF-1 and REX-1 was studied in 52 specimens from
human gonadal development and in 86 samples from TGCT. RESULTS UTF-1 and REX-1 …
BACKGROUND
UTF-1 and REX-1/ZFP42 are transcription factors involved in pluripotency. Because of phenotypic similarities between pluripotent embryonic stem cells and testicular germ cell tumours (TGCT) and the derivation of pluripotent cells from testes, we investigated the expression of UTF-1 and REX-1 during human gonadal development and in TGCT.
METHODS
Expression of UTF-1 and REX-1 was studied in 52 specimens from human gonadal development and in 86 samples from TGCT.
RESULTS
UTF-1 and REX-1 were expressed throughout male gonadal development. In the mature testis, UTF-1 was expressed in spermatogonia, whereas REX-1 was expressed in meiotic cells and, together with OCT-3/4, in primary oocytes. Both UTF-1 and REX-1 were expressed in testicular carcinoma in situ and in TGCT. Contrarily to REX-1, UTF-1 was expressed in all spermatocytic seminomas.
CONCLUSIONS
Unlike other pluripotency markers NANOG and OCT-3/4, UTF-1 and REX-1 are expressed throughout human testes development. The expression pattern indicated that UTF-1 plays a possible role in spermatogonial self-renewal, whereas expression of REX-1 in meiotic cells from both testes and ovary indicate a role in meiosis. UFT-1 and REX-1 are expressed in TGCT and the high abundance of UTF-1 in spermatocytic seminomas is consistent with the hypothesis that this tumour type originates from spermatogonia.
Oxford University Press以上显示的是最相近的搜索结果。 查看全部搜索结果