Overall prevalence rate of Subclinical mastitis in present study was 56.1 per cent. Quarter-wise highest prevalence of subclinical mastitis was recorded in right hind quarter (37.78%) followed by left hind quarter (26.08%), right forequarter (21.73%) and left forequarter (17.39%). Lactation stage-wise analysis indicated more prevalence in 4th to 5th month (70%) followed by 1st to 3rd month of lactation (30%). The study revealed predominance of Staphylococcus, Streptococcus and Escherichia coli as the causative agents of bovine sub clinical mastitis. Overall sensitivity of isolates from sub clinical mastitis in HF cows indicated maximum sensitivity to Gentamicin (93.75%) followed by Chloramphenicol (87.5%) and Ciprofloxacin (75%).