The purpose of this study was to detect of antibodies against Bovine Viral Diarrhea Virus (BVDV) in blood and milk serum samples. With this aim, a total of 192 blood and milk samples were collected from unvaccinated Holstein cows in Kars district of Turkey. Blood and milk serum samples were tested to determine the presence of antibodies against BVDV by commercial indirect enzyme-linked immunosorbent assay (ELISA). In this study, while 172 of blood serum (89.58%) were found to be positive for the presence of BVDV antibodies, 161 of milk serum samples (83.85%) were positive. In addition, 150 (78.12%) both blood and milk serum samples of same cattle were positive for BVDV antibodies. Only 22 (11.45%) blood serum of cattle was detected positive for BVDV, while only 11 (5.73%) milk serum was seropositive. Data obtained from the study showed the presence of BVDV infection in dairy herds in the Kars region and demonstrate that blood serum and milk serum samples might be consistent with one another in the determination of BVDV seroprevalence.