… Therefore, we suggest that in the primer design for SARS-CoV-2, it is more effective to
design the primer targeting position on the stem, at least to make sure that the 3 ' terminal bases …

… a Three-step guidelines for PCR primer design and … each primer set, we designed new
primer sets targeting the RdRP, S, and N genes in the SARS-CoV-2 genome, where each primer …

… SARS-CoV-2 genomes revealed that the new coronavirus … to facilitate real-time mutation
tracking of SARS-CoV-2. This … used for the design of SARS-CoV-2 primer and probe sets by …

… positive and/or negative cases of the new coronavirus. … of SARS-CoV-2-positive samples
using hCOVassay2 primer at … concentrations of quantification control for SARS-CoV-2 (iDT—…

… acid detection of SARS-CoV-2 by quantitative real time … acute respiratory syndrome
coronavirus (SARS-CoV-2, also refers … , quantitative real time polymerase chain reaction (qRT-PCR) …

… public health decisions during the COVID-19 pandemic. … However, despite the availability
of vaccines, emerging new … for publication of quantitative real-time PCR experiments (MIQE) to …

… respiratory syndrome coronavirus 2 (SARS-CoV-2) beginning in … RT-PCR was performed on
an ABI 7500 real-time PCR … World Reference Center for Emerging Viruses and Arboviruses (…

… acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has … provide a general way
to determine other emerging variants. … in a CFX96 Touch real-time PCR detection system (CFX96; …

… of testing capacity persisting during the COVID-19 outbreak, our … shortages, and newly
evolving SARS-CoV-2 variants that could … primer hits in any organisms besides SARS-CoV-2, …

… capabilities for COVID-19 or other emerging pathogens … in silico primer design tools only
serve as a guideline, and primer … step for the CDC SARS-CoV-2 primer sets, we have found that …