Production of paramylon, a β‐1,3‐glucan, by heterotrophic cultivation of Euglena gracilis on a synthetic medium

B Šantek, M Felski, K Friehs, M Lotz… - Engineering in Life …, 2009 - Wiley Online Library
Euglena gracilis was cultivated on a synthetic medium of well‐defined components.
Biomass and paramylon (β‐1, 3‐glucan) concentrations were the most important variables
monitored. Mass production in the bioreactor was carried out following studies of operating
conditions in shaken flasks. E. gracilis grew best at about 30° C and at a low pH of 3. A pH
control was not necessary, although the pH increased considerably at the end of the
cultivation processes. Aeration could be performed at low stirrer frequency. Biomass …

Production of paramylon, a β‐1,3‐glucan, by heterotrophic cultivation of Euglena gracilis on potato liquor

B Šantek, M Felski, K Friehs, M Lotz… - Engineering in Life …, 2010 - Wiley Online Library
Euglena gracilis is shown to be able to grow on potato liquor as the main medium
component leading to an interesting biotechnological product represented by paramylon–a
β‐1, 3‐glucan–and, at the same time, revaluating an otherwise annoying waste stream of
the potato‐starch industry. Paramylon mass fractions of about 75% are obtained for biomass
concentrations of 15 g/L during simple batch cultivation under heterotrophic conditions.
Supplementation of the growth medium with glucose and the vitamins B1 and B12 are …
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