Chronic suppurative otitis media (CSOM) is the biggest public health problem in some populations in the world that can cause morbidity and mortality. Fungus is rare except when there is a super fungal infections of the outer ear canal. The purpose of this study was to know the profile of CSOM patients with positive culture results using KOH examination and Saboroud dextrose agar. The age of CSOM patients most are in the age group 22-31 years old (38.7%), men 50.7% and women was 49.3%. The amount of positive fungal culture was 54.7%, and the culture of the fungus Aspergillus sp was the most (31.4%), consisting of Aspergillus niger (18.7%), Aspergillus fumigatus (8.7%), Aspergillus flavus (4.0%), and Candida sp (24.0%).