Human lens-lipid membranes prepared from the total lipids extracted from clear and
cataractous lens cortexes and nuclei of 61–70-year-old donors by use of a rapid solvent-
exchange method were investigated. The measured cholesterol-to-phospholipid (Chol/PL)
molar ratio in these membranes was 1.8 and 4.4 for cortex and nucleus of clear lenses,
respectively, and 1.14 and 1.45 for cataractous lenses. Properties and organization of the
lipid bilayer were investigated by use of electron paramagnetic resonance spin-labeling …

Purpose: To compare the domain structure and properties of human lens lipid membranes
prepared from the total lipids extracted from the clear and cataractous lens cortex and
nucleus of 61-to 70-year-old donors. This research is important for further studies of intact
human lens membranes, especially for the elucidation of the effect of membrane proteins on
the lateral organization of the lipid bilayer portion of these membranes. Methods: Human
lens lipid membranes were prepared using a rapid solvent exchange method. Their …