The regulation of mRNA transport is a fundamental process for cytoplasmic sorting of
transcripts and spatially controlled translational derepression once properly localized. There
is growing evidence that translation is locally modulated as a result of specific synaptic
inputs. However, the underlying molecular mechanisms that regulate this translational
process are just emerging. We show that KIS, a serine/threonine kinase functionally related
to microtubule dynamics and axon development, interacts with three proteins found in RNA …

MATERIALS AND METHODS Cortical-cell culture, expression vectors, and siRNAs.
Cerebral cortices of embryonic day 14 mouse embryos were dissociated and cultured in
neurobasal medium (Gibco) plus 1% N2, 2% B27, 0.5 mM L-glutamine, and 20 ng/ml brain-
derived neurotrophic factor (BDNF)(Preprotech) on polylysine-laminincoated plates or glass
coverslips at 75,000 cells/cm2. The medium was replaced every 2 days. Mus musculus KIS
(IMAGE ID, 6414877) fused to three copies of the FLAG or hemagglutinin (HA) epitope was …