Proteolytic cleavage and shedding of the bovine prion protein in two cell culture systems
H Zhao, M Klingeborn, M Simonsson, T Linné - Virus research, 2006 - Elsevier
We have compared the processing, turnover and release of bovine PrP (boPrP) in
transfected baby hamster kidney (BHK) and mouse neuroblastoma (N2a) cells. In BHK cells,
boPrP was subjected to two distinct proteolytic cleavage events, the first was mapped
between K121 and H122 generating an N-terminal and a C-terminal PrP fragment.
Transport block experiments, cell surface biotinylation and PIPLC analyses showed that the
bulk of boPrP on the cell surface was the C-terminal fragment and indicated that the first …
transfected baby hamster kidney (BHK) and mouse neuroblastoma (N2a) cells. In BHK cells,
boPrP was subjected to two distinct proteolytic cleavage events, the first was mapped
between K121 and H122 generating an N-terminal and a C-terminal PrP fragment.
Transport block experiments, cell surface biotinylation and PIPLC analyses showed that the
bulk of boPrP on the cell surface was the C-terminal fragment and indicated that the first …
Proteolytic cleavage and shedding of the bovine prion protein in two cell culture systems.
ZHX Zhao HongXing, M Klingeborn, M Simonsson… - 2006 - cabidigitallibrary.org
We have compared the processing, turnover and release of bovine PrP (boPrP) in
transfected baby hamster kidney (BHK) and mouse neuroblastoma (N2a) cells. In BHK cells,
boPrP was subjected to two distinct proteolytic cleavage events, the first was mapped
between K121 and H122 generating an N-terminal and a C-terminal PrP fragment.
Transport block experiments, cell surface biotinylation and PIPLC analyses showed that the
bulk of boPrP on the cell surface was the C-terminal fragment and indicated that the first …
transfected baby hamster kidney (BHK) and mouse neuroblastoma (N2a) cells. In BHK cells,
boPrP was subjected to two distinct proteolytic cleavage events, the first was mapped
between K121 and H122 generating an N-terminal and a C-terminal PrP fragment.
Transport block experiments, cell surface biotinylation and PIPLC analyses showed that the
bulk of boPrP on the cell surface was the C-terminal fragment and indicated that the first …
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