K Fukui, S Uchiyama - The Chemical Record, 2007 - Wiley Online Library
… it is known that each chromosome contains two … humanmetaphasechromosome protein framework using two main methods of collecting, isolating, and identifying humanchromosomal …
H Takata, S Uchiyama, N Nakamura… - Genes to …, 2007 - Wiley Online Library
… , based on our proteomeanalyses together with the previous information, we provide the list of proteins that are essential for the structural organization of metaphasechromosomes. …
… Here we present the first mass spectrometry‐driven proteomeanalysis of metaphase chromosomes prepared from HeLa S3 cells. The preparative method used has been previously …
… accumulating and preparing humanmetaphasechromosomes in sufficient amount to perform their proteomeanalysis. Preparation and separation methods of chromosome proteins are …
S Uchiyama - Chromosome Science, 2009 - jstage.jst.go.jp
… of chromosomes, namely the proteome of chromosomes. Then, … the proteome analyses of humanmetaphasechromosome. … our proteomicanalyses of humanmetaphasechromosomes, …
S Uchiyama, S Kobayashi, H Takata… - … and Genome Research, 2004 - karger.com
… In the present study, we isolated a large amount of humanmetaphasechromosomes using the … have been determined for the proteomeanalysis of constituent proteins of human …
G Sauer, R Korner, A Hanisch, A Ries, EA Nigg… - … & cellular proteomics, 2005 - ASBMB
… Here we describe the purification of human mitotic spindles and their analysis by MS/MS. … This study illustrates the strength of a proteomic approach for the analysis of isolated human …
R Malik, R Lenobel, A Santamaria, A Ries… - Journal of proteome …, 2009 - ACS Publications
… Proteomeanalysis of cells synchronized at late mitosis has, to the best of our knowledge, not previously been described. To ensure specific enrichment at the selected mitotic stages, an …
… An alternative approach for the identification of chromosomal proteins is proteomicanalysis following the purification of vertebrate mitotic chromosomes from cultured cells. This has only …