Proteome analysis of human metaphase chromosomes

S Uchiyama, S Kobayashi, H Takata, T Ishihara… - Journal of Biological …, 2005 - ASBMB
… fiber into metaphase chromosomes. To obtain a global view of the chromosomal proteins,
we performed proteome analyses on three types of isolated human metaphase chromosomes. …

Chromosome protein framework from proteome analysis of isolated human metaphase chromosomes

K Fukui, S Uchiyama - The Chemical Record, 2007 - Wiley Online Library
… it is known that each chromosome contains two … human metaphase chromosome protein
framework using two main methods of collecting, isolating, and identifying human chromosomal

A comparative proteome analysis of human metaphase chromosomes isolated from two different cell lines reveals a set of conserved chromosome‐associated …

H Takata, S Uchiyama, N Nakamura… - Genes to …, 2007 - Wiley Online Library
… , based on our proteome analyses together with the previous information, we provide the
list of proteins that are essential for the structural organization of metaphase chromosomes. …

Proteomic analysis of human metaphase chromosomes reveals topoisomerase II alpha as an Aurora B substrate

C Morrison, AJ Henzing, ON Jensen… - Nucleic acids …, 2002 - academic.oup.com
… Here we present the first mass spectrometry‐driven proteome analysis of metaphase
chromosomes prepared from HeLa S3 cells. The preparative method used has been previously …

Preparation methods of human metaphase chromosomes for their proteome analysis

K Fukui, H Takata, S Uchiyama - Organelle Proteomics, 2008 - Springer
… accumulating and preparing human metaphase chromosomes in sufficient amount to perform
their proteome analysis. Preparation and separation methods of chromosome proteins are …

The proteome of human metaphase chromosomes

S Uchiyama - Chromosome Science, 2009 - jstage.jst.go.jp
… of chromosomes, namely the proteome of chromosomes. Then, … the proteome analyses of
human metaphase chromosome. … our proteomic analyses of human metaphase chromosomes, …

Protein composition of human metaphase chromosomes analyzed by two-dimensional electrophoreses

S Uchiyama, S Kobayashi, H Takata… - … and Genome Research, 2004 - karger.com
… In the present study, we isolated a large amount of human metaphase chromosomes using
the … have been determined for the proteome analysis of constituent proteins of human

Proteome analysis of the human mitotic spindle

G Sauer, R Korner, A Hanisch, A Ries, EA Nigg… - … & cellular proteomics, 2005 - ASBMB
… Here we describe the purification of human mitotic spindles and their analysis by MS/MS. …
This study illustrates the strength of a proteomic approach for the analysis of isolated human

Quantitative analysis of the human spindle phosphoproteome at distinct mitotic stages

R Malik, R Lenobel, A Santamaria, A Ries… - Journal of proteome …, 2009 - ACS Publications
Proteome analysis of cells synchronized at late mitosis has, to the best of our knowledge,
not previously been described. To ensure specific enrichment at the selected mitotic stages, an …

Novel components of human mitotic chromosomes identified by proteomic analysis of the chromosome scaffold fraction

R Gassmann, AJ Henzing, WC Earnshaw - Chromosoma, 2005 - Springer
… An alternative approach for the identification of chromosomal proteins is proteomic analysis
following the purification of vertebrate mitotic chromosomes from cultured cells. This has only …