Protein synthesis and degradation determine the cellular levels of proteins, and their control
hence enables organisms to respond to environmental change. Experimentally, these are
little known proteome parameters; however, recently, SILAC-based mass spectrometry
studies have begun to quantify turnover in the proteomes of cell lines, yeast, and animals.
Here, we present a proteome-scale method to quantify turnover and calculate synthesis and
degradation rate constants of individual proteins in autotrophic organisms such as algae …