Tempe is a traditional Indonesian fermented soybean mostly produced in small industries
and sold locally throughout the country. Studies on the bioactive peptides in tempe are rare.
Here, we studied bioactive peptides in samples from three tempe producers with different
degrees of sanitation. The peptide sub-fractions of tempe from each producer were collected
following water extraction, ultrafiltration (< 3 kDa), gel filtration chromatography, and
reversed phase-high performance liquid chromatography (RP-HPLC) separation followed …