Pseudomonas aeruginosa biofilm inactivation: decreased cell culturability, adhesiveness to surfaces, and biofilm thickness upon high-pressure nonthermal plasma …

AJ Zelaya, G Stough, N Rad… - … on Plasma Science, 2010 - ieeexplore.ieee.org
AJ Zelaya, G Stough, N Rad, K Vandervoort, G Brelles-Mariño
IEEE Transactions on Plasma Science, 2010ieeexplore.ieee.org
Bacterial biofilms are more resilient to standard killing methods than free-living bacteria.
Pseudomonas aeruginosa PAO1 biofilms grown on borosilicate coupons were treated with
gas-discharge plasma for various exposure times. Almost 100% of the cells were inactivated
after a 5-min plasma exposure. Atomic force microscopy was used to image the biofilms and
study their micromechanical properties. Results show that the adhesiveness to borosilicate
and the thickness of the Pseudomonas biofilms are reduced upon plasma treatment.
Bacterial biofilms are more resilient to standard killing methods than free-living bacteria. Pseudomonas aeruginosa PAO1 biofilms grown on borosilicate coupons were treated with gas-discharge plasma for various exposure times. Almost 100% of the cells were inactivated after a 5-min plasma exposure. Atomic force microscopy was used to image the biofilms and study their micromechanical properties. Results show that the adhesiveness to borosilicate and the thickness of the Pseudomonas biofilms are reduced upon plasma treatment.
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