The effect of stitching patterns on the puncture resistance of multiple layers of Twaron fabric was investigated. Fabric samples of two to four fabric layers were stitched with nylon threads using vertical, diagonal, square and diamond patterns. A conical shape probe was used in the puncture resistance tests. The load and extension needed to puncture the fabric samples were recorded. From the results, the puncture loads and extensions increased as the fabric layers increased. It was found that the 4-layer diamond stitched pattern fabric gave slightly higher puncture load and extension in comparisons with other types of stitching patterns. Nevertheless, it can be said that there were no significant difference in the puncture load and extension among the different types of stitching patterns.