ABSTRACT β‐D‐glucosidase has been purified from naringinase of Aspergillus niger to
homogeneity by ammonium sulfate fractionation and chromatographies on
diethylaminoethyl Sepharose, phenyl‐Sepharose and Sephacryl S‐200 HR, with the aid of
prunin used as a specific substrate to detect the β‐D‐glucosidase activity by a high‐
performance liquid chromatography method. The molecular weight of the β‐D‐glucosidase
was determined to about 100 kDa by exclusive gel chromatography and sodium dodecyl …

Aspergillus niger, an isolate of soil contaminated with effluents from cotton ginning mill was
grown in Czapek-Dox medium containing sawdust, Triton-X 100 and urea for production of
an extracellular β-glucosidase. β-Glucosidase enzyme was purified (86-fold) from culture
filtrate of A. niger by employing ammonium sulphate precipitation and gel filtration on
sephadex G-75. The molecular mass of the purified enzyme was estimated to be 95 kDa by
sodium dodecyl sulphate polyacrylamide gel electrophoresis. The enzyme had an optimal …