adenylyl cyclase based on creating a fusion with a small peptide epitope. Using
oligonucleotide technology to encode the peptide epitope we constructed a plasmid that
expressed the fusion protein from the S. cerevisiae alcohol dehydrogenase promoter ADH1.
A monoclonal antibody previously raised against the peptide was used to purify adenylyl
cyclase by affinity chromatography. The purified enzyme appeared to be a multisubunit …