Pyridoxal kinase: a vitamin B6 salvage pathway enzyme from Leishmania donovani
Abstract Pyridoxal kinase (PdxK, EC 2.7. 1.35) is an important enzyme of vitamin B6 salvage
pathway which is required for phosphorylation of B6 vitamers. In the present study, pyridoxal
kinase (pdxK) gene from Leishmania donovani (LdPdxK) was cloned and a 33 kDa protein
was expressed and kinetically characterized. Site-directed mutagenesis was performed to
determine the functional significance of conserved GXGD motif. Mutation of Thr229 to Ala
did not affect the catalytic function of Ld PdxK however Gly228, Gly230 and Asp231 were …
pathway which is required for phosphorylation of B6 vitamers. In the present study, pyridoxal
kinase (pdxK) gene from Leishmania donovani (LdPdxK) was cloned and a 33 kDa protein
was expressed and kinetically characterized. Site-directed mutagenesis was performed to
determine the functional significance of conserved GXGD motif. Mutation of Thr229 to Ala
did not affect the catalytic function of Ld PdxK however Gly228, Gly230 and Asp231 were …
Pyridoxal kinase: a vitamin B6 salvage pathway enzyme from Leishmania donovani.
VK Vinay Kumar, MS Mahesh Sharma, BR Rakesh… - 2018 - cabidigitallibrary.org
Abstract Pyridoxal kinase (PdxK, EC 2.7. 1.35) is an important enzyme of vitamin B6 salvage
pathway which is required for phosphorylation of B6 vitamers. In the present study, pyridoxal
kinase (pdxK) gene from Leishmania donovani (Ld PdxK) was cloned and a 33 kDa protein
was expressed and kinetically characterized. Site-directed mutagenesis was performed to
determine the functional significance of conserved GXGD motif. Mutation of Thr229 to Ala
did not affect the catalytic function of Ld PdxK however Gly228, Gly230 and Asp231 were …
pathway which is required for phosphorylation of B6 vitamers. In the present study, pyridoxal
kinase (pdxK) gene from Leishmania donovani (Ld PdxK) was cloned and a 33 kDa protein
was expressed and kinetically characterized. Site-directed mutagenesis was performed to
determine the functional significance of conserved GXGD motif. Mutation of Thr229 to Ala
did not affect the catalytic function of Ld PdxK however Gly228, Gly230 and Asp231 were …
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