Pyruvate phosphate dikinase (PPDK) was recently reported in trypanosomatids, but its
metabolic function is not yet known. The present work deals with the cellular localization and
the function of the Trypanosoma cruzi enzyme. First, we show by digitonin titration and cell
fractionation that the enzyme was essentially present in the glycosome matrix of the
epimastigote form. Second, we address the issue of the direction of the reaction inside the
glycosome for one part, our bibliographic survey evidenced a quite exergonic ΔG°′(at …