Gold nanorods are extensively used for single-molecule fluorescence enhancement as they
are easy to synthesize, bio-compatible, and provide high light confinement at their
nanometer-sized tips. The current way to estimate fluorescence enhancement relies on
binned time traces or on fluorescence correlation spectroscopy. We report on novel ways to
extract the enhancement factor in a single-molecule enhancement experiment, avoiding the
arbitrary selection of one or a few high-intensity burst (s). These new estimates for the …