Quantitative microvascular hemoglobin mapping using visible light spectroscopic Optical Coherence Tomography

SP Chong, CW Merkle, C Leahy… - Biomedical optics …, 2015 - opg.optica.org
Biomedical optics express, 2015opg.optica.org
Quantification of chromophore concentrations in reflectance mode remains a major
challenge for biomedical optics. Spectroscopic Optical Coherence Tomography (SOCT)
provides depth-resolved spectroscopic information necessary for quantitative analysis of
chromophores, like hemoglobin, but conventional SOCT analysis methods are applicable
only to well-defined specular reflections, which may be absent in highly scattering biological
tissue. Here, by fitting of the dynamic scattering signal spectrum in the OCT angiogram using …
Quantification of chromophore concentrations in reflectance mode remains a major challenge for biomedical optics. Spectroscopic Optical Coherence Tomography (SOCT) provides depth-resolved spectroscopic information necessary for quantitative analysis of chromophores, like hemoglobin, but conventional SOCT analysis methods are applicable only to well-defined specular reflections, which may be absent in highly scattering biological tissue. Here, by fitting of the dynamic scattering signal spectrum in the OCT angiogram using a forward model of light propagation, we quantitatively determine hemoglobin concentrations directly. Importantly, this methodology enables mapping of both oxygen saturation and total hemoglobin concentration, or alternatively, oxyhemoglobin and deoxyhemoglobin concentration, simultaneously. Quantification was verified by ex vivo blood measurements at various pO_2 and hematocrit levels. Imaging results from the rodent brain and retina are presented. Confounds including noise and scattering, as well as potential clinical applications, are discussed.
opg.optica.org
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