[HTML][HTML] Quantitative proteomic analysis of cellular responses to a designed amino acid feed in a monoclonal antibody producing Chinese hamster ovary cell line
F Torkashvand, F Mahboudi, M Vossoughi… - Iranian Biomedical …, 2018 - ncbi.nlm.nih.gov
Iranian Biomedical Journal, 2018•ncbi.nlm.nih.gov
Background: Chinese hamster ovary (CHO) cell line is considered as the most common cell
line in the biopharmaceutical industry because of its capability in performing efficient post-
translational modifications and producing the recombinant proteins, which are similar to
natural human proteins. The optimization of the upstream process via different feed
strategies has a great impact on the target molecule expression and yield. Methods: To
determine and understand the molecular events beneath the feed effects on the CHO cell, a …
line in the biopharmaceutical industry because of its capability in performing efficient post-
translational modifications and producing the recombinant proteins, which are similar to
natural human proteins. The optimization of the upstream process via different feed
strategies has a great impact on the target molecule expression and yield. Methods: To
determine and understand the molecular events beneath the feed effects on the CHO cell, a …
Abstract
Background:
Chinese hamster ovary (CHO) cell line is considered as the most common cell line in the biopharmaceutical industry because of its capability in performing efficient post-translational modifications and producing the recombinant proteins, which are similar to natural human proteins. The optimization of the upstream process via different feed strategies has a great impact on the target molecule expression and yield.
Methods:
To determine and understand the molecular events beneath the feed effects on the CHO cell, a label-free quantitative proteomic analysis was applied. The proteome changes followed by the addition of a designed amino acid feed to the monoclonal antibody producing CHO cell line culture medium were investigated.
Results:
The glutathione synthesis, the negative regulation of the programmed cell death, proteasomal catabolic process, and the endosomal transport pathway were up-regulated in the group fed with a designed amino acid feed compared to the control group.
Conclusion:
Our findings could be helpful to identify new targets for metabolic engineering.
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