sequencing of MPXV and rapid tracing of genetic variants will benefit disease diagnosis and
control. It is a significant challenge but necessary to optimize the strategy and application of
rapid full-length genome identification and to track variations of MPXV in clinical specimens
with low viral loads, as it is one of the DNA viruses with the largest genome and the most AT-
biased, and has a significant number of tandem repeats. Here we evaluated the …