Recognizing the reagent microbiome

MC de Goffau, S Lager, SJ Salter, J Wagner… - Nature …, 2018 - nature.com
… of the reagent contamination profile and between-batch variation of such profiles are two of
the most powerful tools that can be used to recognize reagent … can be reagent contamination …

Developing whole cell standards for the microbiome field

C Sergaki, S Anwar, M Fritzsche, R Mate, RJ Francis… - Microbiome, 2022 - Springer
reagent that is specific for the gut microbiome and can be used to evaluate and benchmark
DNA extractions in microbiome … where biases occur in microbiome pipelines. In the future, we …

Optimizing methods and dodging pitfalls in microbiome research

D Kim, CE Hofstaedter, C Zhao, L Mattei, C Tanes… - Microbiome, 2017 - Springer
… Different batches of DNA extraction kit reagents can be a significant source of variation for
… Problems with contamination were well recognized even before the era of deep sequencing …

Microbiome testing in Europe: navigating analytical, ethical and regulatory challenges

J Rodriguez, M Cordaillat-Simmons, N Badalato… - Microbiome, 2024 - Springer
microbiome research has flourished and has drawn attention from both healthcare professionals
and general consumers as the human microbiome is now recognized … a reagent or …

Issues and current standards of controls in microbiome research

BVH Hornung, RD Zwittink… - FEMS microbiology …, 2019 - academic.oup.com
… In microbiome research, however, controls have not been included in the … publications from
the 2018 issues of Microbiome and the ISME journal (… Recognizing the reagent microbiome

Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome

Z Han, PS Thuy-Boun, W Pfeiffer, VF Vartabedian… - Scientific Reports, 2021 - nature.com
… SA is recognized by immunoinhibitory sialic acid-binding … of these microbes from complex
microbiome samples may reveal … the concentration of either labeling reagent only amplified the …

Quantifying and understanding well-to-well contamination in microbiome research

JJ Minich, JG Sanders, A Amir, G Humphrey… - …, 2019 - Am Soc Microbiol
… Such contamination may arise from laboratory or reagent sources or from physical … other
samples rather than reagent contaminants. IMPORTANCE Microbiome research has uncovered …

RETRACTED ARTICLE: Microbiome analyses of blood and tissues suggest cancer diagnostic approach

GD Poore, E Kopylova, Q Zhu, C Carpenter… - Nature, 2020 - nature.com
… We recognize the importance of measuring and … reagent tubes during sample processing,
we next attempted to pair a microbial blacklist at the genus level that used similar reagents and/…

Identification and removal of contaminating microbial DNA from PCR reagents: impact on low‐biomass microbiome analyses

LF Stinson, JA Keelan, MS Payne - Letters in applied …, 2019 - academic.oup.com
… treatment of PCR reagents should be explored as a simple and effective way of reducing
contamination in low‐biomass microbiome studies and … Recognizing the reagent microbiome

Considerations for optimizing microbiome analysis using a marker gene

J De la Cuesta-Zuluaga, JS Escobar - Frontiers in nutrition, 2016 - frontiersin.org
… During the last decade, the study of the gut microbiome has … of study and is now recognized
as an object of intervention (1… of technical controls of the reagents to be used (storage media, …