Regulation of cyclooxygenase expression in the kidney by dietary salt intake
T Yang, I Singh, H Pham, D Sun… - American Journal …, 1998 - journals.physiology.org
The present studies were undertaken to determine the effect of dietary salt intake on the
renal expression of cyclooxygenase-1 (COX-1) and-2 (COX-2). Protein levels were
assessed by Western blotting, and mRNA expression was assessed by reverse transcription-
polymerase chain reaction (RT-PCR) on cDNA prepared from kidney regions, dissected
nephron segments, and cultured renal cells. Both isoforms were expressed at high levels in
inner medulla (IM), with low levels detected in outer medulla and cortex. COX-1 mRNA was …
renal expression of cyclooxygenase-1 (COX-1) and-2 (COX-2). Protein levels were
assessed by Western blotting, and mRNA expression was assessed by reverse transcription-
polymerase chain reaction (RT-PCR) on cDNA prepared from kidney regions, dissected
nephron segments, and cultured renal cells. Both isoforms were expressed at high levels in
inner medulla (IM), with low levels detected in outer medulla and cortex. COX-1 mRNA was …
Regulation of cyclooxygenase expression in the kidney by dietary salt intake.
YTX Yang TianXin, IS Inderjit Singh, PH Pham Hang… - 1998 - cabidigitallibrary.org
The effect of dietary salt intake (0.03%(low salt group) or 3% NaCl+ 0.45% NaCl as drinking
fluid (high salt group) for 7 days) on the renal expression of cyclooxygenase-1 (COX-1) and-
2 (COX-2) was examined in rats. Protein levels were assessed by Western blotting, and
mRNA expression was assessed by reverse transcription-polymerase chain reaction on
cDNA prepared from kidney regions, dissected nephron segments, and cultured renal cells.
Both isoforms were expressed at high levels in inner medulla (IM), with low levels detected …
fluid (high salt group) for 7 days) on the renal expression of cyclooxygenase-1 (COX-1) and-
2 (COX-2) was examined in rats. Protein levels were assessed by Western blotting, and
mRNA expression was assessed by reverse transcription-polymerase chain reaction on
cDNA prepared from kidney regions, dissected nephron segments, and cultured renal cells.
Both isoforms were expressed at high levels in inner medulla (IM), with low levels detected …
以上显示的是最相近的搜索结果。 查看全部搜索结果