Regulation of matrix metalloproteinase-3 gene expression in inflammation: A molecular study
Materials and Methods: Lipopolysaccharide-induced murine macrophage-like RAW 264.7
cell lines were obtained and maintained in Dulbecco's modified Eagle's medium plus 10%
fetal bovine serum under optimal temperatures. Primers used were MMP-3 forward primer,
MMP-3 reverse primer, and glyceraldehyde-3-phosphate dehydrogenase forward primer
and glyceraldehyde-3-phosphate reverse primer. Total RNA was isolated, the sample was
prepared, and electrophoresis was performed. The first strand of cDNA was synthesized and …
cell lines were obtained and maintained in Dulbecco's modified Eagle's medium plus 10%
fetal bovine serum under optimal temperatures. Primers used were MMP-3 forward primer,
MMP-3 reverse primer, and glyceraldehyde-3-phosphate dehydrogenase forward primer
and glyceraldehyde-3-phosphate reverse primer. Total RNA was isolated, the sample was
prepared, and electrophoresis was performed. The first strand of cDNA was synthesized and …
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