Regulation of the cyanide-resistant alternative oxidase of plant mitochondria: identification of the cysteine residue involved in α-keto acid stimulation and intersubunit …
DM Rhoads, AL Umbach, CR Sweet… - Journal of Biological …, 1998 - ASBMB
The cyanide-resistant alternative oxidase of plant mitochondria is a homodimeric protein
whose activity can be regulated by a redox-sensitive intersubunit sulfhydryl/disulfide system
and by α-keto acids. After determining that theArabidopsis alternative oxidase possesses the
redox-sensitive sulfhydryl/disulfide system, site-directed mutagenesis of an Arabidopsis
cDNA clone was used to individually change the two conserved Cys residues, Cys-128 and
Cys-78, to Ala. Using diamide oxidation and chemical cross-linking of the protein expressed …
whose activity can be regulated by a redox-sensitive intersubunit sulfhydryl/disulfide system
and by α-keto acids. After determining that theArabidopsis alternative oxidase possesses the
redox-sensitive sulfhydryl/disulfide system, site-directed mutagenesis of an Arabidopsis
cDNA clone was used to individually change the two conserved Cys residues, Cys-128 and
Cys-78, to Ala. Using diamide oxidation and chemical cross-linking of the protein expressed …
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