A study was conducted to examine the influence of hydraulic retention time (HRT) and solid retention time (SRT) on the removal of tetracycline in the activated sludge processes. Two lab-scale sequencing batch reactors (SBRs) were operated to simulate the activated sludge process. One SBR was spiked with 250 μg/L tetracycline, while the other SBR was evaluated at tetracycline concentrations found in the influent of the wastewater treatment plant (WWTP) where the activated sludge was obtained. The concentrations of tetracyclines in the influent of the WWTP ranged from 0.1 to 0.6 μg/L. Three different operating conditions were applied during the study (phase 1HRT:  24 h and SRT:  10 days; phase 2HRT:  7.4 h and SRT:  10 days; and phase 3HRT:  7.4 h and SRT:  3 days). The removal efficiency of tetracycline in phase 3 (78.4 ± 7.1%) was significantly lower than that observed in phase 1 (86.4 ± 8.7%) and phase 2 (85.1 ± 5.4%) at the 95% confidence level. The reduction of SRT in phase 3 while maintaining a constant HRT decreased tetracycline removal efficiency. Sorption kinetics reached equilibrium within 24 h. Batch equilibrium experiments yielded an adsorption coefficient (K_ads) of 8400 ± 500 mL/g and a desorption coefficient (K_des) of 22 600 ± 2200 mL/g. No evidence of biodegradation for tetracycline was observed during the biodegradability test, and sorption was found to be the principal removal mechanism of tetracycline in activated sludge.