We read with great interest the article published by Zuo et al, which highlighted the presence
of SARS-CoV-2 RNA in stool samples during active and convalescence phases of COVID-
19 infection. 1 However, no study has reported the presence of viral antigens within GI and
hepatic organs during the convalescent phase.Using conventional immunohistochemistry,
we detected SARS-CoV-2 nucleocapsid protein (NP) in the colon, appendix, ileum,
haemorrhoid, liver, gallbladder and lymph nodes (figure 1A–K) from five patients who …

Abstract Residual SARS-CoV-2 RNA has been detected in stool samples and
gastrointestinal tissues during the convalescence phase of COVID-19 infection. This raises
concern for persistence of SARS-CoV-2 virus particles and faecal-oral transmissibility in
recovered COVID-19 patients. Using multiplex immunohistochemistry, we unexpectedly
detected SARS-CoV-2 viral antigens in intestinal and liver tissues, in surgical samples
obtained from two patients who recovered from COVID-19. We further validated the …