Resurrection of an alpha-1, 3-galactosyltransferase gene-targeted miniature pig by recloning using postmortem ear skin fibroblasts
KS Ahn, YJ Kim, M Kim, BH Lee, SY Heo, MJ Kang… - Theriogenology, 2011 - Elsevier
Animals with a targeted disruption of genes can be produced by somatic cell nuclear transfer
(SCNT). However, difficulties in clonal selection of somatic cells with a targeted mutation
often result in heterogeneous nuclear donor cells, including gene-targeted and non-targeted
cells, and impose a risk of producing undesired wildtype cloned animals after SCNT. In
addition, the efficiency of cloning by SCNT has remained extremely low. Most cloned
embryos die in utero, and the few that develop to term show a high incidence of postnatal …
(SCNT). However, difficulties in clonal selection of somatic cells with a targeted mutation
often result in heterogeneous nuclear donor cells, including gene-targeted and non-targeted
cells, and impose a risk of producing undesired wildtype cloned animals after SCNT. In
addition, the efficiency of cloning by SCNT has remained extremely low. Most cloned
embryos die in utero, and the few that develop to term show a high incidence of postnatal …
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