Reversible Ponceau staining as a loading control alternative to actin in Western blots
Analytical biochemistry, 2010•Elsevier
It is becoming standard practice to measure a housekeeping gene, typically actin, in
Western blots, as it is the rule in RNA blots. We have applied reversible Ponceau staining to
check equal loading of gels and measured actin in parallel under different conditions. Our
results show that densitometric analysis is comparable with both techniques. Therefore,
routine quantitation of Ponceau staining before antibody probing is validated as an
alternative to actin blotting.
Western blots, as it is the rule in RNA blots. We have applied reversible Ponceau staining to
check equal loading of gels and measured actin in parallel under different conditions. Our
results show that densitometric analysis is comparable with both techniques. Therefore,
routine quantitation of Ponceau staining before antibody probing is validated as an
alternative to actin blotting.
It is becoming standard practice to measure a housekeeping gene, typically actin, in Western blots, as it is the rule in RNA blots. We have applied reversible Ponceau staining to check equal loading of gels and measured actin in parallel under different conditions. Our results show that densitometric analysis is comparable with both techniques. Therefore, routine quantitation of Ponceau staining before antibody probing is validated as an alternative to actin blotting.
Elsevier
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