l-aspartic acid (L-AA) based MOFs were prepared from acetate, chloride, nitrate, and sulfate salts of Cu(II) ions as L-AA-Cu(II)-A,-C,-N,-S, respectively with 96.7 ​± ​2.4, 89.8 ​± ​3.1, 92.1 ​± ​1.5, 74.6 ​± ​5.2 ​m²/g, surface areas. L-AA-Cu(II)-MOFs in the same order induced 0.43 ​± ​0.25%, 0.94 ​± ​0.24%, 0.91 ​± ​0.40%, 1.18 ​± ​0.10% hemolysis, all being <2%, and blood clotting indices of ~90% and can be considered nonhemolytic and non-coagulative at 1 ​μg/mL concentration. L-AA-Cu(II)-A MOFs exhibited 86.3 ​± ​0.2, and 92.4 ​± ​0.6% α-Glucosidase inhibitory activities at 1.0 and 10.0 ​μg/mL concentrations, respectively. Moreover, L-AA-Cu(II)-S MOFs had effective antimicrobial activities against E. coli (ATCC-8739), and S. aureus (ATCC-6538) with MIC values of 0.63 ​mg/mL and 1.25 ​mg/mL for C. albicans (ATCC-10231). L-AA-Cu(II) MOFs synthesized herein with hemocompatible, antimicrobial and antidiabetic properties prompt interesting possibilities for both industrial and biomedical applications.