Role of the Pif1-PCNA complex in Pol δ-dependent strand displacement DNA synthesis and break-induced replication
The S. cerevisiae Pif1 helicase functions with DNA polymerase (Pol) δ in DNA synthesis
during break-induced replication (BIR), a conserved pathway responsible for replication fork
repair and telomere recombination. Pif1 interacts with the DNA polymerase processivity
clamp PCNA, but the functional significance of the Pif1-PCNA complex remains to be
elucidated. Here, we solve the crystal structure of PCNA in complex with a non-canonical
PCNA-interacting motif in Pif1. The structure guides the construction of a Pif1 mutant that is …
during break-induced replication (BIR), a conserved pathway responsible for replication fork
repair and telomere recombination. Pif1 interacts with the DNA polymerase processivity
clamp PCNA, but the functional significance of the Pif1-PCNA complex remains to be
elucidated. Here, we solve the crystal structure of PCNA in complex with a non-canonical
PCNA-interacting motif in Pif1. The structure guides the construction of a Pif1 mutant that is …