Toilet training is the process of teaching toddlers to control bladder and bowel. It is a challenging process full of successes and failures. Aim: to assess Saudi mothers' knowledge, attitudes and practices regarding toddlers toilet training readiness. A descriptive study that was conducted at the Pediatric Ward. A purposive sample of 100 mothers was involved. Four tools were used to assess mothers socio-demographic data, general and specific knowledge, attitudes and practices. The majority of the mothers (88%) had poor knowledge,(97%), poor attitudes and (76%) average practice. Conclusion, there was significant correlations between mothers' knowledge, attitudes and practices. Recommendations: develop an educational Program for mothers about toilet training readiness and conducting the same study on mothers of developmentally delayed children.