Efficient transgene expression in recipient cells constitutes the primary step in gene therapy.
However, random integration in host genome comprises too many uncertainties. Our study
presents a strategy combining bioinformatics and functional verification to find transgene
integration sites in pig genome. Using an in silico approach, we screen out two candidate
sites, namely, Pifs302 and Pifs501, located in actively transcribed intergenic regions with
low nucleosome formation potential and without potential non-coding RNAs. After …

Efficient transgene expression in recipient cells constitutes the primary step in gene therapy.
However, random integration in host genome comprises too many uncertainties. Our study
presents a strategy combining bioinformatics and functional verification to find transgene
integration sites in pig genome. Using an in silico approach, we screen out two candidate
sites, namely, Pifs302 and Pifs501, located in actively transcribed intergenic regions with
low nucleosome formation potential and without potential non-coding RNAs. After …