Selection and Assessment of Reference Genes for Quantitative PCR Normalization in Migratory Locust Locusta migratoria (Orthoptera: Acrididae)

Q Yang, Z Li, J Cao, S Zhang, H Zhang, X Wu… - PLoS …, 2014 - journals.plos.org
… appropriate reference genes for qPCR analyses, as all the selected reference genes exhibited
… Furthermore, the best recommended reference genes were also different. According to …

Selection of Reference Genes for RT-qPCR Analysis in the Monarch Butterfly, Danaus plexippus (L.), a Migrating Bio-Indicator

H Pan, X Yang, K Bidne, RL Hellmich, BD Siegfried… - PloS one, 2015 - journals.plos.org
… -quantitative polymerase chain reaction (RT-qPCR) is a … that two reference genes are required
for reliable normalization … ; three reference genes are required for reliable normalization in …

Screening potential reference genes for quantitative real-time PCR analysis in the oriental armyworm, Mythimna separata

HB Li, CG Dai, CR Zhang, YF He, HY Ran, SH Chen - PloS one, 2018 - journals.plos.org
migrant and resident M. separata [22]. Furthermore, Chang et al. … three reference genes were
required for normalizing gene … two reference genes were sufficient to normalize expression …

Selection of reference genes for the normalization of RT-qPCR data in gene expression studies in insects: a systematic review

J Lü, C Yang, Y Zhang, H Pan - Frontiers in physiology, 2018 - frontiersin.org
… It is considered the ideal reference gene for RT-qPCR analysis and has been investigated
most frequently (Figure 3). For example, previous studies have shown that the expression of …

Gene expression studies of reference genes for quantitative real-time PCR: an overview in insects

M Shakeel, A Rodriguez, UB Tahir, F Jin - Biotechnology letters, 2018 - Springer
genes, is the usual method of choice for normalizing gene expression. Although reference
genes are used to normalize target geneanalysis is to be conducted and a reference gene

… Evaluation of Suitable Reference Genes for Normalization of MicroRNA Expression in Helicoverpa armigera (Lepidoptera: Noctuidae) Using Quantitative Real-Time …

Y Yang, Z Li, J Cao, Y Li, H Li, Q Yang… - Journal of Insect …, 2017 - academic.oup.com
… armigera, selecting uniform and suitable reference genes … In this paper, 10 relatively stable
candidate reference genes, … sequencing, were selected to normalize qRT-PCR data, and 1 …

Reference genes in real-time PCR

B Kozera, M Rapacz - Journal of applied genetics, 2013 - Springer
… Since during selection of the most stable normalizing genegene selection, primer design
and robust statistical analysis. This is … conducted studies of bad data migration and spread. To …

Reliable reference genes for gene expression analyses under the hypomagnetic field in a migratory insect

Y Zhang, L Zeng, Y Wei, M Zhang, W Pan… - Frontiers in …, 2022 - frontiersin.org
quantitative polymerase chain reaction (RT-qPCR) has been … Expression stability evaluation
of candidate reference genes … females normalized to the reference genes evaluated in this …

Selection and Evaluation of Reference Genes for qRT-PCR in Spodoptera frugiperda (Lepidoptera: Noctuidae)

S Han, Q Qin, D Wang, Y Zhou, Y He - Insects, 2021 - mdpi.com
Quantitative real-time PCR (qRT-PCR) is an important … Normalization of the data relies on
stable reference genes. The … an important invasive and migratory pest that seriously threatens …

Selection of housekeeping genes for gene expression studies in larvae from flatfish using real-time PCR

C Infante, MP Matsuoka, E Asensio, JP Cañavate… - BMC Molecular …, 2008 - Springer
genes as useful reference genes for accurate normalization … begins very late with the migration
of the left eye about 80 DAH… Twelve candidate HKGs for gene expression analysis during …