The stable carbon isotope compositions (δ¹³C) of tropical lake sediments and soils have been used to reconstruct the occurrence of prehistoric maize cultivation and its relative importance through time. This study assesses some of the possible variables affecting the response of lake sediment bulk organic carbon isotope (δ¹³C_TOC) values to variations in the scale of prehistoric maize cultivation and the potential of this proxy to yield quantitative estimates of the scale of prehistoric maize agriculture in small tropical watersheds. High resolution analyses of δ¹³C_TOC values, maize pollen concentrations, and mineral influx were conducted on sediments deposited during a ∼220year period of prehistoric maize agriculture in the watershed of Laguna Castilla, a small lake in the mid-elevations of the Cordillera Central, Dominican Republic. Close correspondence between δ¹³C_TOC values and maize pollen concentrations in the Laguna Castilla sediment record indicates a close relationship between the isotopic values and the scale of prehistoric maize cultivation. Correlations between the δ¹³C_TOC signature and mineral influx indicate that the isotope record is also sensitive to variations in allochthonous carbon delivery. This study establishes that sedimentary δ¹³C_TOC values can provide a highly sensitive proxy of the spatial scale of prehistoric maize agriculture in small tropical watersheds, but emphasizes the need for a better understanding of sediment dynamics and carbon cycling in anthropogenically modified landscapes before this proxy can be widely employed in diverse archaeological settings.