Toxoplasmosis is a global zoonotic infection caused by Toxoplasma gondii. Various studies have earlier associated T. gondii with serious health hazards including abortion, still birth, anorexia, anemia etc. Toxoplasmosis could be serious medical condition and potentially harmful during the first trimester of pregnancy in women. The aim of this study was to evaluate seropositivity and possible risk factors of toxoplasmosis in pregnant women. A total of 180 blood samples were randomly collected to determine the seroprevalence rate of antibodies against T. gondii in the pregnant women of District Kohat using Enzyme Linked Immunosorbent Assay (ELISA). The overall seroprevalence was 14.4%(n= 26/180) among the pregnant women. A significant difference (P< 0.05) of seropositivity was observed statistically between inhabitants of rural 21.27% and urban areas 6.97% respectively. An increased consumption of undercooked meat 21.53% and unwashed vegetables or fruit 25% were observed in serologically positive women. Multivariate analysis indicated that the risk for T. gondii infection increased with age, lower educational level, populous living conditions and soil-related occupations, although, in subset analyses, risk categories varied with ethnicity.