The purpose of this paper was to investigate and describe opinions about the significance of operational art, submitted by military officers who studied the career courses at the Centre for Security and Military Strategic Studies, the University of Defense in the Czech Republic in the years 2014-2016. The data were collected using the qualitative and quantitative research methods. The case study model encompassing three research inquiries, completed with a structural questionnaire were used for this research. The questionnaire included 11 questions/statements that were complementary with the content of the case study. The research was conducted with the sample of the courses participants, which comprised 101 students from different military units within the organizational structure of the Czech Armed Forces. The results indicate that there are different opinions about significance and understanding of the operational art theory, its significance, and its skillful application in practice.