Simultaneous detection of six stone fruit viruses by non-isotopic molecular hybridization using a unique riboprobe or 'polyprobe'
MC Herranz, JA Sanchez-Navarro, F Aparicio… - Journal of Virological …, 2005 - Elsevier
A new strategy for the simultaneous detection of plant viruses by molecular hybridization has
been developed. Two, four or six viral sequences were fused in tandem and transcribed to
render unique riboprobes and designated as 'polyprobes'. The 'polyprobe four'(poly 4)
covered the four ilarviruses affecting stone fruit trees including apple mosaic virus (ApMV),
prunus necrotic ringspot virus (PNRSV), prune dwarf virus (PDV), and American plum line
pattern virus (APLPV) whereas the 'polyprobe two'(poly 2) was designed to detect …
been developed. Two, four or six viral sequences were fused in tandem and transcribed to
render unique riboprobes and designated as 'polyprobes'. The 'polyprobe four'(poly 4)
covered the four ilarviruses affecting stone fruit trees including apple mosaic virus (ApMV),
prunus necrotic ringspot virus (PNRSV), prune dwarf virus (PDV), and American plum line
pattern virus (APLPV) whereas the 'polyprobe two'(poly 2) was designed to detect …
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