Simultaneous generation of many RNA-seq libraries in a single reaction

AA Shishkin, G Giannoukos, A Kucukural, D Ciulla… - Nature …, 2015 - nature.com
… Here we report RNAtag-Seq, a method for generating a single RNA-seq library containing
large numbers of RNA samples that are barcoded and pooled before library construction (…

Multiplexed single-cell RNA-seq via transient barcoding for simultaneous expression profiling of various drug perturbations

D Shin, W Lee, JH Lee, D Bang - Science advances, 2019 - science.org
… for simultaneous single-cell transcriptome profiling for multiple … were kinase inhibitors,
including several BCR-ABL–targeting … reactions of the Nextera XT DNA Library Preparation Kit. …

[PDF][PDF] RNA-seq using next generation sequencing

DC Corney - Mater Methods, 2013 - researchgate.net
… produce one read in a single-end sequencing reaction, … libraries by introducing barcodes
during library preparation. This allows simultaneous sequencing of multiple libraries in a single

A highly multiplexed and sensitive RNA-seq protocol for simultaneous analysis of host and pathogen transcriptomes

R Avraham, N Haseley, A Fan, Z Bloom-Ackermann… - Nature protocols, 2016 - nature.com
… protocol for simultaneous analysis of host and bacterial transcripts by RNA-seq. Importantly,
… Add 80 μl of binding buffer and 80 μl of EtOH (100%) to each reaction (binding buffer and …

Simul-seq: combined DNA and RNA sequencing for whole-genome and transcriptome profiling

JA Reuter, DV Spacek, RK Pai, MP Snyder - Nature methods, 2016 - nature.com
… Our simultaneous DNA and RNA sequencing method, Simul-… In parallel, we also prepared
independent RNA-seq librariesreactions were assembled using diluted Simul-seq libraries (…

NASC-seq monitors RNA synthesis in single cells

GJ Hendriks, LA Jung, AJM Larsson… - Nature …, 2019 - nature.com
Single-cell RNA-seq libraries were constructed using a … NASC-seq measures global RNA
replacement rates in single cells. a … Still, we believe the simultaneous measure of two cellular …

Massively parallel and time-resolved RNA sequencing in single cells with scNT-seq

Q Qiu, P Hu, X Qiu, KW Govek, PG Cámara, H Wu - Nature methods, 2020 - nature.com
… To identify the optimal reaction conditions on barcoded beads, we explored two chemical …
simultaneous handling of multiple samples. Third, scNT-seq costs <$0.50 per cell for library

MULTI-seq: sample multiplexing for single-cell RNA sequencing using lipid-tagged indices

CS McGinnis, DM Patterson, J Winkler, DN Conrad… - Nature …, 2019 - nature.com
… to 3.2× reaction volumes and added 1.8× reaction volumes of … library preparation PCR (95
C, 5′; 98 C, 15′; 60 C, 30′; 72 C, 30′; eight cycles; 72 C, 1′; 4 C hold). Each reaction

RNASeq methods for transcriptome analysis

R Hrdlickova, M Toloue, B Tian - Wiley Interdisciplinary …, 2017 - Wiley Online Library
… a quick reaction (… single-cell RNA-Seq to detect over-amplified products. In addition, barcoding
is typically used to label cells, enabling simultaneous preparation of libraries from multiple

SIDR: simultaneous isolation and parallel sequencing of genomic DNA and total RNA from single cells

KY Han, KT Kim, JG Joung, DS Son, YJ Kim… - Genome …, 2018 - genome.cshlp.org
… that various sequencing metrics for DR-Seq WGS and RNA-seq … the other because of
simultaneous amplification of DNA and … of single cells, a reduction of the reaction volumes from …