A method based on femtosecond two-photon excitation has been developed for simultaneous visualization of interference-free fluorescence of H and O atoms in turbulent flames. This work shows pioneering results on single-shot simultaneous imaging of these radicals under non-stationary flame conditions. The fluorescence signal, showing the distribution of H and O radicals in premixed CH_4/O_2 flames was investigated for equivalence ratios ranging from ϕ = 0.8 to ϕ = 1.3. The images have been quantified through calibration measurements and indicate single-shot detection limits on the order of a few percent. Experimental profiles have also been compared with profiles from flame simulations, showing similar trends.