Solid-phase enzyme modification via affinity chromatography
In the present study antileukemic enzyme l-asparaginase (ASNase) and catalase (as a
model enzyme) were modified in solid-phase with activated polyethylene glycol (PEG2) by
using ligand-immobilized affinity column systems l-asparagine-Sepharose CL-4B and
Procion red-Sepharose CL-4B, respectively. Studies on change of specific activity with
modification time showed negligible differences between batches of modified catalase.
Modification of ASNase for 1 h resulted in 50.2% recovery of the specific activity and the …
model enzyme) were modified in solid-phase with activated polyethylene glycol (PEG2) by
using ligand-immobilized affinity column systems l-asparagine-Sepharose CL-4B and
Procion red-Sepharose CL-4B, respectively. Studies on change of specific activity with
modification time showed negligible differences between batches of modified catalase.
Modification of ASNase for 1 h resulted in 50.2% recovery of the specific activity and the …
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