G protein-coupled receptors (GPCRs) are the largest class of molecules involved in signal
transduction across cell membranes and are major drug targets. Since GPCRs are integral
membrane proteins, their structure and function are modulated by membrane lipids. In
particular, membrane cholesterol is an important lipid in the context of GPCR function.
Solubilization of integral membrane proteins is a process in which the proteins and lipids in
native membranes are dissociated in the presence of a suitable amphiphilic detergent …