Recent advances in Cross-Technology Communication (CTC) enable the coexistence and collaboration among heterogeneous wireless devices operating in the same ISM band (e.g., Wi-Fi, ZigBee, and Bluetooth in 2.4 GHz). However, state-of-the-art CTC schemes are vulnerable to spoofing attacks since there is no practice authentication mechanism yet. This paper proposes a scheme to enable the spoofing attack detection for CTC in heterogeneous wireless networks by using physical layer information. First, we propose a model to detect ZigBee packets and measure the corresponding Received Signal Strength (RSS) on Wi-Fi devices. Then, we design a collaborative mechanism between Wi-Fi and ZigBee devices to detect the spoofing attack. Finally, we implement and evaluate our methods through experiments on commercial off-the- shelf (COTS) Wi-Fi and ZigBee devices. Our results show that it is possible to measure the RSS of ZigBee packets on Wi-Fi device and detect spoofing attack with both a high detection rate and a low false positive rate in heterogeneous wireless networks.