Aquaporin 0 (AQP0) is a transmembrane protein specific to the eye lens, involved as a water
carrier across the lipid membranes. During eye lens maturation, AQP0s are truncated by
proteolytic cleavage. We investigate in this work the capability of truncated AQP0 to conduct
water across membranes. We developed a method to accurately determine water
permeability across lipid membranes and across proteins from the deflation under osmotic
pressure of giant unilamellar vesicles (GUVs) deposited on an adhesive substrate. Using …

Vr: Reduced volume of the osmotic shock R (t) Radius of the GUV at time t A (t): Area of the
adhering patch at time t 𝑹𝒑𝒂𝒕𝒄𝒉: Radius of the adhering patch V (t): Volume of the GUV at
time t 𝑹𝒗𝟎, 𝑹𝒗: Radius of the GUV before and during adhesion 𝑹𝒗𝒇: Radius of the GUV at
equilibrium after the osmotic shock A0, 𝑨𝒇: GUV surface before and at equilibrium after the
osmotic shock S0, S: Surface of the GUV before and during adhesion 𝑽𝟎, 𝑽𝒇: GUV volume
before the shock and at equilibrium after the shock γ0, γ: Surface tension of the GUVbefore …