Static and dynamic culture of human endothelial cells encapsulated inside alginate-gelatin microspheres
This study aimed to explore the angiogenesis potential of human endothelial cells
encapsulated inside alginate-gelatin microspheres under static and dynamic culture
systems after 7 days. Human umbilical vein endothelial cells were encapsulated inside
alginate (1%) and gelatin (1.2%) using an electrostatic encapsulation method. Cells were
incubated for 7 days in vitro. The cell survival rate was measured using the MTT assay. The
expression of VEGFR-2 and von Willebrand factor genes was studied by real-time PCR …
encapsulated inside alginate-gelatin microspheres under static and dynamic culture
systems after 7 days. Human umbilical vein endothelial cells were encapsulated inside
alginate (1%) and gelatin (1.2%) using an electrostatic encapsulation method. Cells were
incubated for 7 days in vitro. The cell survival rate was measured using the MTT assay. The
expression of VEGFR-2 and von Willebrand factor genes was studied by real-time PCR …
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