Cellulase-producing thermophilic bacteria was isolated from West Coast hot spring
(Rajapur, Ratnagiri District of Maharashtra (Lat. 16° 38′ 42 ″N; long. 73° 31′ 53 ″E) and
identified as Bacillus licheniformis. Out of seven variables in the cellulase production
medium, four variables such as carboxy methyl cellulose (CMC), calcium chloride, Tween-
20, and temperature were screened through Plackett–Burman design and further optimized …