Stress-related genes distinctly expressed in unfertilized wheat ovaries under both normal and water deficit conditions whereas differed in fertilized ovaries
In this study, a proteomic approach was utilized to identify differentially accumulated proteins
in developing wheat ovaries before and after fertilization and in response to water deficit.
Proteins were extracted, quantified, and resolved by 2-DE at pH 4–7. Statistical analysis of
spot intensity was performed by using principal component analysis and samples were
clustered by using Euclidean distance. In total, 136 differentially accumulated protein spots
representing 88 unique proteins were successfully identified by MALDI-TOF/TOF MS. Under …
in developing wheat ovaries before and after fertilization and in response to water deficit.
Proteins were extracted, quantified, and resolved by 2-DE at pH 4–7. Statistical analysis of
spot intensity was performed by using principal component analysis and samples were
clustered by using Euclidean distance. In total, 136 differentially accumulated protein spots
representing 88 unique proteins were successfully identified by MALDI-TOF/TOF MS. Under …
Stress-related genes distinctly expressed in unfertilized wheat ovaries under both normal and water deficit conditions whereas differed in fertilized ovaries.
QYJ Qin YaJuan, SWL Song WanLu… - 2014 - cabidigitallibrary.org
In this study, a proteomic approach was utilized to identify differentially accumulated proteins
in developing wheat ovaries before and after fertilization and in response to water deficit.
Proteins were extracted, quantified, and resolved by 2-DE at pH 4-7. Statistical analysis of
spot intensity was performed by using principal component analysis and samples were
clustered by using Euclidean distance. In total, 136 differentially accumulated protein spots
representing 88 unique proteins were successfully identified by MALDI-TOF/TOF MS. Under …
in developing wheat ovaries before and after fertilization and in response to water deficit.
Proteins were extracted, quantified, and resolved by 2-DE at pH 4-7. Statistical analysis of
spot intensity was performed by using principal component analysis and samples were
clustered by using Euclidean distance. In total, 136 differentially accumulated protein spots
representing 88 unique proteins were successfully identified by MALDI-TOF/TOF MS. Under …
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