Structural and functional characterization of the promoter region of the mouse c-Ki-ras gene
EK Hoffman, SP Trusko, N Freeman… - Molecular and cellular …, 1987 - Taylor & Francis
EK Hoffman, SP Trusko, N Freeman, DL George
Molecular and cellular biology, 1987•Taylor & FrancisWe isolated and characterized the 5′ region of the mouse c-Ki-ras gene, including a 5′
untranslated exon (exon 0). These studies used genetic material from Y1 mouse
adrenocortical tumor cells in which the c-Ki-ras gene is amplified and overexpressed. Our
data demonstrate that transcription initiates at multiple sites, predicting size heterogeneity at
the 5′ ends of the c-Ki-ras mRNAs. Using transient expression assays, we identified a
genomic fragment within the 5′ region which exhibits bidirectional promoter activity.
untranslated exon (exon 0). These studies used genetic material from Y1 mouse
adrenocortical tumor cells in which the c-Ki-ras gene is amplified and overexpressed. Our
data demonstrate that transcription initiates at multiple sites, predicting size heterogeneity at
the 5′ ends of the c-Ki-ras mRNAs. Using transient expression assays, we identified a
genomic fragment within the 5′ region which exhibits bidirectional promoter activity.
We isolated and characterized the 5′ region of the mouse c-Ki-ras gene, including a 5′ untranslated exon (exon 0). These studies used genetic material from Y1 mouse adrenocortical tumor cells in which the c-Ki-ras gene is amplified and overexpressed. Our data demonstrate that transcription initiates at multiple sites, predicting size heterogeneity at the 5′ ends of the c-Ki-ras mRNAs. Using transient expression assays, we identified a genomic fragment within the 5′ region which exhibits bidirectional promoter activity.
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