Structural basis of exo-β-mannanase activity in the GH2 family
The classical microbial strategy for depolymerization of β-mannan polysaccharides involves
the synergistic action of at least two enzymes, endo-1, 4-β-mannanases and β-
mannosidases. In this work, we describe the first exo-β-mannanase from the GH2 family,
isolated from Xanthomonas axonopodis pv. citri (XacMan2A), which can efficiently hydrolyze
both manno-oligosaccharides and β-mannan into mannose. It represents a valuable
process simplification in the microbial carbon uptake that could be of potential industrial …
the synergistic action of at least two enzymes, endo-1, 4-β-mannanases and β-
mannosidases. In this work, we describe the first exo-β-mannanase from the GH2 family,
isolated from Xanthomonas axonopodis pv. citri (XacMan2A), which can efficiently hydrolyze
both manno-oligosaccharides and β-mannan into mannose. It represents a valuable
process simplification in the microbial carbon uptake that could be of potential industrial …
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